Lactose Broth

Lactose Broth is a liquid medium used for the detection and/or verification of the presence of Salmonella and other coliform organisms in water, foods, dairy, and pharmaceutical products. This medium is also used as a pre-enrichment in the analysis of food, milk products, and water for Salmonella.

Principles Lactose Broth also provides good growth characteristics for coliforms and fermentation is observed by gas bubbles present in an inverted fermentation tube. The concentration of media prepared for the test should be adjusted to accommodate a volume of sample greater than 10mL. Pancreatic Digest of Gelatin and Beef Extract provide nitrogenous, carbon and vitamin sources, being Lactose the fermentable carbohydrate.