Accuris SmartDrop™ Nano Spectrophotometers

The Accuris SmartDrop™ Nano Spectrophotometers provides quick and easy quantification of small samples. Utilizing a small sample volume eliminates the need for dilutions, providing more accurate results.

A full color, 7” touchscreen with icon-driven menus and preprogrammed protocols gets users up and running quickly. No external computer is required. Each unit features a quartz pedestal onto which a drop of sample is placed. The arm is lowered and the drop is stretched into a liquid column and specified length for spectral analysis. DNA , RNA, and protein concentrations and purity numbers are displayed in a matter of seconds. The resulting data can be printed, saved in the SmartDrop’s onboard memory, or exported via USB as an Excel file. All SmartDrop models include an integrated printer. For OD600 readings, each model includes a cuvette port and two optical glass cuvettes.

Three SmartDrop models are available to meet a range of applications and budgets:

SmartDrop L is the affordable choice for laboratories needing to determine nucleic acid and protein concentrations and purity of samples. The LED light source and silicon photocell sensor provide readings at 260 and 280nm. Samples from 1.0 to 2.0µl and loaded directed on the pedestal. Results are provided in about five (5) seconds. The software automatically calculates and displays 260/280 ratios.

• Ideal for basic DNA/RNA/protein quantification and purity
• Affordable LED and silicon detector technology
• Fixed 0.5mm path length, detect up to 2500ng/µl of dsDNA

SmartDropX is a full spectrum UV-Vis spectrophotometer requiring 0.5 to 2.0µl of sample. In addition to nucleic acid and protein calculations, the software has preset programs for BCA, Bradford, and Lowry assays. A full spectrum analysis can be performed in about six (6) seconds, aiding in the identification of unknowns. Up to five (5) specific points on the spectrum can be selected for colorimetric readings output. The long-life Xenon lamp source requires no warmup and is paired with a highly sensitive CCD detector. To provide the most accurate results, the SmartDrop X utilizes two (2) path lengths.

• Full-spectrum absorbance scans from 200 to 800nm
• Quickly calculates and displays 260/280 and 260/230 ratios
• Long-life Xenon lamp with precision monochromator
• Auto-ranging path lengths of 0.2mm and 1.0mm
• Detect up to 4,500 ng/µl of ds DNA without dilution

The SmartDrop XF is the top-of-the-line spectrophotometer and can perform not only UV-Vis readings in the range of 200-800nm but also fluorescence readings. Like the SmartDrop X, the SmartDrop XF features predefined programs for nucleic acids, proteins, and various colorimetric assays. The patented, motorized system gently stretches the sample column and allows for three different path lengths, providing accurate readings of samples from 0.5 to 2.0µl without dilution. The integrated fluorometer yields more accurate, sensitive, and selective quantitation of nucleic acids. The blue light channel (460nm) is useful for detection of dyes such as PicoGreen and RiboGreen as well as GFP and fluorescein. UV, green, and red channel models are available upon request.

Accuris DNA Quantification Kits

Accuris DNA Quantification Kits are designed for accurate fluorescent quantification of dsDNA using instruments such as the Qubit®, SmartDrop™, and microplate readers. The reagents are highly selective for double-stranded DNA (dsDNA); quantification results will not be skewed by single-stranded DNA (ssDNA), RNA, protein, or nucleotides in a sample. Included with each kit are a concentrated fluorescent assay reagent, dilution buffer, and ready-to-use DNA standards. The assay reagent is diluted using the buffer provided, sample is added (1µL to 20µL), and the concentration determined using an appropriate fluorometer instrument that utilizes blue excitation (460±20 nm) and measures green fluorescence (525-570nm).

The Accuris High Sensitivity Kit provides an accurate method to quantify low concentration ds DNA samples. The assay kit is designed to be accurate for initial DNA sample concentrations of 0.005–120 ng/µL, providing a detection range of 0.1–120ng.

The Accuris Broad Range Kit provides an accurate method to quantify dsDNA samples that have a broad concentration range starting at 0.2 ng/µL. The assay kit is designed to be accurate for initial DNA sample concentrations of 0.2 to 2,000 ng/µL, providing a detection range of 4–2,000 ng.