An Exclusive Weber Product: AllergenControl™ LFD Detection Kits (Microbiologique)

Any food business with a HACCP or HARPC plan should have a Food Allergen Management plan

Reasons why AllergenControl kits are special

• Added security of Competitive line to prevent false negatives. Some Lateral Flow Devices (LFD’s) can give false negatives when high levels of food allergen are present in the sample. This system uses a third line to overcome this problem
• Avoid waste. Kits are available for either environmental surface tests or food product tests. Buy only what you need.
• Wide range of kits available for specificity.
• Doesn’t need refrigeration – store between 36° to 77°F (2° to 25°C).
• Simple to use – no reconstitution of reagents – simply extract and run.
• Samples are tested against supplied known controls.
• Heat-stable protein allergens are detected.
• Custom Competitive Pricing™ is available. Just ask.

Assay principles

The AllergenControl residue LFD features polyclonal antibodies directed against targeted proteins configured in both sandwich and competitive formats. To operate the kit, the sample is first subjected to a short extraction step. The sample extract is then directly applied to the lateral flow device sample port, where it is allowed to migrate across the reagent zone thus enabling visualization of the Sandwich Test Line (T) and Competitive Test Line (0) results. In addition, the reagent zone includes a Procedural Control Line (C) to minimize the potential for false negative outcomes.

Interpretation of the Test

The reagent zone contains 3 distinct print lines that must be considered in a coordinated manner when interpreting the test lines. These lines include the Sandwich Test Line (T), the Competitive Test Line (0), and the Control Line (C). The Sandwich Test Line (T) will become clearly visible at the LOD value defined for the kit and continue to strengthen in intensity with increasing target analyte concentration up to a point where it will then start to fade and eventually disappear at high analyte levels. The Competitive Test Line (0) will be intense if the sample is negative for the target analyte and fade with increasing amount of analyte, disappearing completely at high analyte levels before the Sandwich Test Line does. This feature allows the operator to distinguish between samples with none or low levels of target analyte and those with high levels. The Competitive Test Line also enables detection of target analyte that is highly hydrolyzed and poorly detected by the Sandwich Test Line. Failure of the Procedural Control Line (C) to appear denotes an invalid test, requiring repeat testing.

Performance characteristics
Verified to avoid cross-reactivity
Limit of Detection: as shown below
1 year shelf life from date of manufacture

Environmental Kit Test Time:
Hands on time: 5 minutes - Total test time: 20 minutes at 95°C incubation; 35 minutes at 60°C incubation

Food Kit Test Time:
Hands on time: 10 minutes - Total test time: 25 minutes at 95°C incubation; 35 minutes at 60°C incubation

SEE DOCUMENTS TAB FOR REQUIRED SUPPLIES AND EQUIPMENT THAT ARE NOT SUPPLIED

Additional residue kits are available by request.
All kits come in either a food or environmental format.
Almond, Almond Milk, Brazil Nut, Cashew, Cashew Milk, Crustacean, Hazelnut, Macadamia, Mustard, Pistachio, Sesame, and Walnut/Pecan.