D-Glucose/Fructose UniFlex Enzymatic Test Kit (Unitech)

D-Glucose/Fructose UniFlex Reagent (=GF-F): An enzymatic test kit for determination of both D-glucose and D-fructose (reducing sugars) in foods, beverages and other samples.

Contains ready-to-use Buffer solution and two enzyme suspensions (Hexokinase/G6PDH and PGI), permitting independent determinations of D-glucose and D-fructose. Also contains D-fructose and D-glucose standards. The Fructose Standard is of particular benefit in monitoring the (slower) PGI mediated reaction.

Reducing sugars are the predominant soluble components of soft fruits, with sucrose in low amounts. D-glucose and D-fructose are the predominant reducing sugars in grape and other fruit juices. The ratio of glucose to fructose in mature grapes is “1”, but ranges from 0.74 - 1.12 according to variety, maturity and fermentation conditions. In ‘stuck fermentations’, D-fructose often predominates, since D-glucose is the preferred energy source for yeast.