OnSite III Allergen Lateral Flow Tests - Casein

OnSite® III Allergen Lateral Flow Tests – Casein is designed for use in the qualitative determination of casein residues obtained from sampling foods (solids, powders, and liquids) and surfaces. Allergies to cow’s milk are one of the most common food allergies, affecting roughly 3% of the population. Consequently, numerous countries require that food labels clearly indicate milk content, Casein is the major protein in milk (80% of total milk proteins) and constitutes a major allergen of clinical importance. Casein is selectively added or present in certain foods. To assist the food industry in establishing effective food safety practices, this rapid test kit can reliably detect Casein residues in food and on surfaces and equipment.

The Casein OnSite® III Allergen Kits consist of lateral flow devices (LFDs) that can be used to rapidly detect allergens in foods, rinse water and environmental surfaces (see link below to order). The LFDs are configured using antibodies that have been raised against the target allergen. To operate the kit, the sample is collected using the supplied scoop and then extracted for 2 min by shaking at room temperature. The tube is allowed to settle for 5 min by resting it. Thereafter, the sample extract is applied to the LFD using a dropper supplied with the kit. The fluid is wicked across the reagent zone, which includes a procedural control line (C), an overload line (O), and a test line (T). The inclusion of an overload line (O) is critical for testing foods that have high analyte contamination to prevent misinterpretation of false negative test outcomes.

Key Features

• Limit of Detection: Detects casein from cow’s milk. down to 1.0 ppm in food and rinse water and down to 1.0 µg/100 cm2 on environmental surfaces.
• Assay Time: ~ 20 min.
• Cross-Reactivity: Cross-reactivity with kidney bean, low level raw fish.
• Kit Components: OnSite® III LFD Devices (10), Single-Use Dropper (10), Breakaway Swabs (10), Pre-Measured OnSite® LFD Extraction Buffer (10), Instructions for use
• The test outcome is interpreted by visualizing the appearance of three lines at 15 min.
• Environmental surface testing requires separate purchase of the OnSite® Allergen Accessory Kit

The test is intended for laboratory use only and should only be performed by trained personnel.