OnSite III Allergen Lateral Flow Tests - Cashew

OnSite® III Allergen Lateral Flow Tests – Cashew is designed for use in the qualitative determination of cashew nut residues obtained from sampling foods (solids, powders, and liquids) and on surfaces. The Cashew (Anacardium occidentale) belongs to a group of foods, commonly refer to as “tree nuts”. This group of foods is a significant source of allergens, and cashews are a frequent cause of food allergy. Consequently, there is a need for a sensitive and reliable test for the detection of specific tree nut allergens in foods. Any such test must be able to detect a heat stable protein component, otherwise false negative results could occur with samples that have been highly heat treated.

The Cashew OnSite® III Allergen Kits consist of lateral flow devices (LFDs) that can be used to rapidly detect allergens in foods, rinse water and environmental surfaces (see link below to order). The LFDs are configured using antibodies that have been raised against the target allergen. To operate the kit, the sample is collected using the supplied scoop and then extracted for 2 min by shaking at room temperature. The tube is allowed to settle for 5 min by resting it. Thereafter, the sample extract is applied to the LFD using a dropper supplied with the kit. The fluid is wicked across the reagent zone, which includes a procedural control line (C), an overload line (O), and a test line (T). The inclusion of an overload line (O) is critical for testing foods that have high analyte contamination to prevent misinterpretation of false negative test outcomes.

Key Features

• Limit of Detection: Detects cashew allergens down to 1.0 ppm in food and rinse water and down to 1.0 µg/100 cm2 on environmental surfaces.
• Assay Time: ~ 20 min.
• Cross-Reactivity: Cross-reactivity with pistachio.
• Kit Components: OnSite® III LFD Devices (10), Single-Use Dropper (10), Breakaway Swabs (10), Pre-Measured OnSite® LFD Extraction Buffer (10), Instructions for Use
• The test outcome is interpreted by visualizing the appearance of three lines at 15 min.
• Environmental surface testing requires separate purchase of the OnSite® Allergen Accessory Kit

The test is intended for laboratory use only and should only be performed by trained personnel.