SimPlate® for Heterotrophic Plate Count (IDEXX)

The US EPA has approved SimPlate for compliance testing of drinking water. It is a new testing method for heterotrophic plate count that eliminates the need for medium preparation and produces easy to use results. SimPlate has significant benefits compared to traditional pour plates:

Easy

• No agar preparation
• Easy to read
• 738 per plate Most Probable Number (MPN) counting range minimizes need for dilutions. (In contrast, traditional standard methods agar plates can only count up to 300 CFU before reaching the TNTC - too numerous to count - level.)
  
  

Rapid

• Instant media preparation
• Less than 2 minutes hands-on time
• Reduced read time.
  
  

Accurate

• Correlates with US EPA-approved pour plate method* using plate count agar and 48-hour, 35°C incubation**
• Simplified procedure minimizes chance of contamination
• Less subjective interpretation than pour plate.
  
  

Economical

• Less equipment to purchase and maintain
• Increased productivity saves labor costs
• Minimal Quality Control (QC).
  
  

Simple

• Add 10 mL sample to media vessel
• Pour contents of tube onto center of plate base
• Cover plate with lid and swirl
• Pour off excess
• Invert and incubate for 48 hours
• Read under UV light.
  
  

American Public Health Association Standard Methods for Examination of Water and Wastewater,19th Ed. 1995, A.D. Eaton, L.S. Clesceri, A.E. Greenberg (eds.), American Public Health Association, Washington, DC.
*Data on file at IDEXX Laboratories, Inc.

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